23 Jul Vegetable leaf vein specific promoter and use thereof
Abstract
The invention discloses a vegetable leaf vein specific promoter and use thereof. With a nucleotide sequence shown by the sequence table 1, the promoter can promote the expression of a target gene in vegetable leaf veins. In the invention, a VS promoter sequence is cloned, and a VS promoter is proved to have unique vein specific activity in arabidopsis thaliana by detecting the expression levels of a ‘VS-promoter-GUS’ fusion gene in organs of the transgenic arabidopsis thaliana; and meanwhile, the transformation of micro tomato Micro-Tom by the ‘VS-promoter-GUS’ fusion gene further proves that the ‘VS-promoter-GUS’ fusion gene is specifically expressed only in the veins of transgenic tomatoes. The promoter of the invention is used to construct a ‘promoter-target gene to be expressed’ fusion gene, and through phytotransformation transgenic plants in which the target genes are specifically expressed in veins can be obtained. The promoter can be used for studying vegetable vascular tissue differentiation, solute translocation mechanism and the like and has an important value in study and use of plant genetic engineering.
Year: 2010
Country: CN
Doc No: 101880666