23 Jul USES OF OLIGONUCLEOTIDES STIMULATORY OF THE MESENCHYMAL STEM CELL PROLIFERATION
Abstract
Oligonucleotides having the ability to greatly stimulate the proliferation of pluripotent mesenchymal stem cells ‘in vitro’ and ‘in vivo’ of animals, including humans, are disclosed. These oligonucleotides can be used in a wide range of clinical procedures such as (1) regeneration of mesenchymal tissues which have been damaged through acute injury, abnormal genetic expression or acquired disease by inoculation of the ODNs of this invention; (2) treatment of a host with damaged mesenchymal tissue by removal of small aliquots of bone marrow, isolation of their mesenchymal stem cells and treatment of the damaged tissue with MSCs culture-expanded by incubation with one or more of the ODNs of this invention combined with a biocompatible carrier suitable for delivering the MSCs to the damaged body site(s); (3) production ‘in vitro’ of various mesenchymal tissues by directed differentiation of the MSCs culture-expanded by incubation with one or more of the ODNs of this invention, to replace and restore tissue damage or defects with say ‘in vitro’ obtained mesenchymal tissues combined with a biocompatible carrier suitable for delivering the ‘in vitro’ produced tissues to the damaged body site(s); and (4) treatment of a host with abnormal genetic expression with MSCs culture-expanded by incubation with one or more of the ODNs of this invention and transformed by genetic engineering procedures to express a protein able to replace the genetic deffect.
Year: 2008
Country: KR
Doc No: 20080019046