23 Jul Tissue engineering vessel and preparation method and application thereof
Abstract
The invention relates to a tissue engineering vessel and a preparation method and an application thereof. The tissue engineering vessel uses a tubular gelatin/polycaprolactone electricity texture membrane as a support, and smooth muscle cells are evenly distributed in the support. The preparation method includes: a) spreading disinfected gelatin/polycaprolactone materials in a culture dish; b) collecting umbilical artery smooth muscle cells, evenly inoculating the umbilical artery smooth muscle cells on the surface of the gelatin/polycaprolactone electricity texture membrane to culture over the night, and forming a cell material composite electricity texture membrane; and c) twisting the cell material composite electricity texture membrane on a polyvinyl chloride (PVC) tube for culturing, and finally forming the tubular tissue engineering vessel. Cells of the tissue engineering vessel prepared by the method are evenly distributed, simultaneously the tissue engineering vessel has the advantages of being good in elasticity, moderate in hardness and compact in tissue structure, having smooth inner walls and abundant collagenous fibers, and being clear in self tissue boundary and the like. The characteristics of the tissue engineering vessel are close to that of living body vessels.
Year: 2013
Country: CN
Doc No: 103230309