23 Jul Tissue engineering skin constructed by sodium alginate hydrogel scaffold and preparation method thereof
Abstract
The invention discloses tissue engineering skin constructed by a sodium alginate hydrogel scaffold. The tissue engineering skin comprises a cuticular layer and a corium layer from top to bottom, wherein the cuticular layer is a layer of seed cells positioned above the corium layer; and the corium layer comprises seed cells and a sodium alginate hydrogel scaffold; the seed cells are positioned in the surface and body of the sodium alginate hydrogel scaffold to form a skin appendage. Since the hydrogel scaffold cross-linked network contains a great amount of moisture, can be used for supplying nutrition to cells, and is crosslinked with EGF to adjust growth and differentiation of cells, so that the hydrogel scaffold can be used for well simulating tissue-like physical and spatial structures essential to cell growth, a three-dimensional cultural space of cells can be provided, culture and function maintenance of stratum basale epidermidis cells can be benefited, and a tissue engineering skin substitute with biological activity can be constructed. When the tissue engineering skin is applied to a wound surface, and recovery of a normal tissue structure of the wound is benefited.
Year: 2016
Country: CN
Doc No: 106110402