23 Jul Tissue engineering myocardium bioreactor constructed by pouring, perfusion and pulsation combination
Abstract
The invention relates to a tissue engineering myocardium bioreactor constructed by pouring, perfusion and pulsation combination, belonging to the technical field of tissue engineering and biological engineering manufacture. The bioreactor comprises a cell pouring generator, a perfusion module, a pulsation generator, a culture module and a control module; the cell pouring generator, the perfusion module and the pulsation generator are communicated with the culture module through pipelines; and the culture module comprises a pulsation cavity, a pulsation effect soft tube with one closed end and a perfusion cavity, and the perfusion module and the pulsation generator carry out perfusion culture and pulsation training. The invention can improve cardiomyocytes planting depth and simulate myocardium in vivo environment, and carries out culture to obtain a tissue engineering myocardium with larger thickness and autonomously beating capacity. The bioreactor is used for realizing that the tissue engineering myocardium construction carries out perfusion culture and pulsation training simultaneously or separately on cell perfusion and tissue culture stages in a cell planting stage.
Year: 2010
Country: CN
Doc No: 101824382