23 Jul Tissue engineering microencapsulated hepatocyte and preparation and application thereof
Abstract
The invention discloses a tissue engineering microencapsulated hepatocyte and a preparation method thereof, belonging to the field of animal cell tissue engineering. The preparation method comprises the following steps of: adding hepatocytes and vascular endothelial cells in the quantity ratio of 1: (0.5-1) into water solution of sodium alginate for mixing to prepare a cell suspension, wherein the mass percentage concentration of the water solution of sodium alginate is 1.5-2; and then preparing sodium alginate-polylysine-sodium alginate microencapsulated hepatocyte by adopting a high-voltage static microcapsule generator. The invention also discloses the application of the tissue engineering microencapsulated hepatocyte in preparing hepatic failure medicaments; the prepared microencapsulated hepatocyte has the characteristics of small volume, high biocompatibility and favorable permeability, is suitably used as a vector for cell transplantation, and can exert in a host body for a long term so as to avoid the side effects caused by repeatedly using chemical medicaments; in addition, the microencapsulated hepatocyte has simple preparation process, thereby being suitable for large-batch production.
Year: 2010
Country: CN
Doc No: 101717767