23 Jul Tissue engineering lymph node model and construction method thereof
Abstract
The invention discloses a tissue engineering lymph node model and a construction method thereof and belongs to the technical field of tissue engineering and regenerative medicine. The tissue engineering lymph node model comprises lymphocytes separated from lymphoid tissues and a collagen-Matrigel scaffold. The construction method for the tissue engineering lymph node model comprises the following steps of: preparing lymphocyte suspension and primary generation mouse fibroblast cell suspension; and compounding mixed cell suspension and the collagen-Matrigel scaffold, and culturing in a mold subjected to static stretching to obtain the tissue engineering lymph node model. The scaffold system subjected to static stretching can better simulate in-vivo environment and support the growth and proliferation of the lymphocytes, secretion of cell factors and the expression of surface markers, and has important significance for applying the tissue engineering lymph node model to immunotoxicity evaluation of biological materials, immune medicine screening and the like. The construction method has the advantages of simple operating process and mild implementation conditions.
Year: 2011
Country: CN
Doc No: 102228719