23 Jul Tissue engineering human corneal stroma carrier bracket and preparation method thereof
Abstract
The invention relates to a method for preparing a tissue engineering human corneal stroma carrier bracket from seawater fish collagen. The method comprises the following steps: completely dissolving non-enzymolysis codfish skin collagen freeze-dried powder into hydrochloric acid; centrifuging and removing precipitate; filling supernate into culture board pores respectively and freeze-drying; adding a 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide/N-hydroxysuccinimide crosslinking agent containing chondroitin sulfate and crosslinking; stabilizing the crosslinking effect by using an alkaline solution of Na2HPO4; cleaning by using 2M of NaCl, 1M of NaCl and distilled water sequentially; and freeze-drying to obtain the tissue engineering human corneal stroma carrier bracket. The process is scientific and reasonable; the prepared carrier bracket can be applied to mass production; heavy demand on the carrier brackets due to large-scale reconstruction of tissue engineering human corneal stromata can be met; conditions are created for sight recovery of blind people suffering from human corneal stroma diseases through clinical corneal transplantation; and the preparation cost of the carrier bracket and the application cost of the carrier bracket to in vitro reconstruction of the tissue engineering human corneal stroma and clinical treatment are low.
Year: 2012
Country: CN
Doc No: 102552975