23 Jul Tissue engineering cartilage composite scaffold and preparation method thereof
Abstract
The invention belongs to the field of cartilage tissue engineering. The invention discloses a tissue engineering cartilage composite scaffold, which is constructed based on an extracellular matrix-derived cartilage micro-tissue, and a preparation method of the composite scaffold. Specifically, a cartilage extracellular matrix-derived micro-carrier is prepared from fresh cartilage through such processes as wet pulverization, sieving, decellularizing and the like; and the prepared micro-carrier can be used as a seed cell amplification vector. By virtue of a bioreactor, cartilage cells are rapidly amplified by the micro-carrier and the phenotype of the cartilage cells is kept; and meanwhile, the induced differentiation of stem cells towards the cartilage is also promoted and the cartilage micro-tissue is formed. By filling the cartilage micro-tissue in pores of a three-dimensional porous scaffold which is good in mechanical performance, the tissue engineering cartilage composite scaffold is constructed. The composite scaffold is good in mechanical performance, rich in natural cartilage extracellular matrix components and conducive to the induced differentiation of the stem cells towards the cartilage, and the composite scaffold is capable of providing a good microenvironment for the growth of seed cells and is expected to accelerate the in vivo construction speed of the tissue engineering cartilage.
Year: 2016
Country: CN
Doc No: 105288737