23 Jul Tissue engineering bone blood vessel radiography method and blood vessel intubation tube
Abstract
The invention relates to a tissue engineering bone blood vessel radiography method. The method includes the steps of firstly, blending a blood vessel radiography agent; secondly, conducting blood vessel tube intubation; thirdly, conducting blood vessel radiography; fourthly, putting an animal body in a refrigerator at 0-4 DEG C overnight, conducting CT scanning or taking a sample from a tissue engineering bone and putting the sample in formalin to be fixed for MicroCT scanning and analyzing. The method has the advantages that blood vessel casting agent lead oxide and a gelatin solution used inthe human anatomy field are adopted, a solution blended according to the ratio is directly injected into animal blood vessels, operation is simple, the bone formation situation and vascularization situation can be displayed on a CT three-dimensional image at the same time without decalcification, and the research on the relationship between bone formation and vascularization in the bone tissue engineering field is facilitated; a protruding structure is arranged in a blood vessel intubation tube, the disengagement of the intubation tube in the blood vessel radiography process is effectively prevented, and the stable injection of the radiography agent is facilitated.
Year: 2018
Country: CN
Doc No: 108937989