23 Jul Tissue engineering bionic liver lobe structure based on 3D printing of living cells and preparation method thereof
Abstract
The invention discloses a tissue engineering bionic liver lobe structure based on 3D printing of living cells and a preparation method thereof. Firstly, biological information of human body liver lobes is collected, and inputted into a computer for high biomimetic modeling; then, material separated printing and material-cell mixed printing output are performed with a hydrogel material and cells for constructing liver, bile duct epithelium, blood vessel endothelium, smooth muscle and fibroblast and the like required for the human liver lobes by a biological 3D printer, wherein a hollow part inblood vessels and bile ducts is printed by a casting agent; after all the hydrogel materials are solidified, the casting agent in the blood vessels and bile ducts are removed by means of enzymes or chelating reactions or temperature control or illumination and the like, and a vascular system and a bile duct system in the artificial bile duct-containing liver lobes are constructed. The circulationfeeding of the vascular system of the bionic liver lobes and gas exchange are achieved by connection of main blood vessels at two ends of the vascular system and a delivery pipe of a culture solution.The long-term survival and biological function of the bionic liver lobes in vitro and in vivo are achieved.
Year: 2019
Country: CN
Doc No: 110403731