23 Jul Tissue culture rapid propagation method for cucurbits
Abstract
The invention provides a tissue culture rapid propagation method for cucurbits, and relates to the technical field of tissue culturing for plants. The method comprises the following steps that 1), cucurbit seeds without hulls are disinfected and inoculated to the surface of an aseptic seedling solid culture medium for aseptic seedling culturing, and aseptic seedlings are obtained; 2), cotyledonarynodes of the aseptic seedlings are peeled and inoculated in a multiple shoot induction culture medium for induction culturing, and multiple shoots are obtained; 3), the multiple shoots are separatedand inoculated in a rooting culture medium for rooting culture, and tissue culture rapid propagation seedlings of cucurbits are obtained. By utilizing the method, within two months, a large number ofgrown cucurbit seedlings with consistent inheritable characters are obtained; the highest multiplication coefficient can reach 2.27, and the highest rooting rate can reach 95%. The method provides method basis for cell engineering of the cucurbits and transgenosis breeding improvement and provides reference for large-scale production of high-quality cucurbit stock seedlings.
Year: 2019
Country: CN
Doc No: 109566420