23 Jul Tissue culture method for increasing regeneration rate of wheat immature embryos
Abstract
The invention discloses a tissue culture method for increasing the regeneration rate of wheat immature embryos. The tissue culture method disclosed by the invention comprises the following steps: (1) putting the wheat immature embryos to be cultured on a callus induction culture medium for culturing, so as to obtain embryogenic calluses, wherein the callus induction culture medium contains arabinogalactan proteins (AGP) with the final concentration of 50-200mg/L; (2) transferring the embryogenic calluses to a differentiation culture medium for culturing, so as to obtain regenerated wheat plants. According to the method, the condition that the regeneration rate of the wheat immature embryos is increased obviously is found through adding AGP to the SD2 callus induction culture medium, and AGP is effective to wheat varieties, easy to regenerate, such as YM18 and JD18 and wheat varieties, difficult to regenerate, such as JM22 and NC4, so that the method has an important significance in improvement on good wheat varieties by means of genetic engineering and cell engineering.
Year: 2013
Country: CN
Doc No: 103444532