23 Jul Tissue culture method for increasing plant regeneration rate of wheat immature embryos
Abstract
The invention discloses a tissue culture method for increasing the regeneration rate of wheat immature embryos. The tissue culture method disclosed by the invention comprises the following steps: (1) putting the wheat immature embryos to be cultured on a callus induction culture medium for culturing, so as to obtain embryogenic calluses, wherein the callus induction culture medium contains hydrogen peroxide (H2O2) with a volume fraction of 0.00015-0.0003%; (2) transferring the embryogenic calluses to a differentiation culture medium for culturing, so as to obtain regenerated wheat plants. According to the method, the condition that the regeneration rate of the wheat immature embryos is increased obviously is found through adding H2O2 to the SD2 callus induction culture medium, and H2O2 is effective to wheat varieties, easy to regenerate, such as YM18, JD18 and CB031 and wheat varieties, difficult to regenerate, such as JM22, NC4 and YM20, so that the method has an important significance in improvement on good wheat varieties by means of genetic engineering and cell engineering.
Year: 2013
Country: CN
Doc No: 103444533