23 Jul Tissue culture and rapid propagation method and application of aquatic plant nymphoides indica
Abstract
The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation method and application of aquatic plant nymphoides indica. Themethod comprises the following steps: acquiring a detoxification parent, amplifying cluster buds, rooting, inducing a complete plant, strengthening the seedlings, transplanting. For the large-scale propagation of the nymphoides indica under an indoor artificial condition, the tissue culture and rapid propagation method is not susceptible to the influence of natural environmental factors; the sterile parent is acquired by virtue of multiple decontamination detoxification means, and the high-efficient propagation of the cluster buds and the induction of developed root systems can be realized byadjusting the content of MS, cane sugar and hormones. By adopting the tissue culture and rapid propagation method, the cost is low, the effectiveness is fast, and the strong and detoxified nymphoidesindica high-quality tissue culture seedlings with consistent specification can be produced in batched in a short time. The obtained seedlings can be applied to the indoor aquarium ornamental layout, outdoor wetland park construction and large-sized wetland ecological restoration engineering, and can effectively solve the problem of the market shortage of the nymphoides indica seedlings in China atpresent.
Year: 2018
Country: CN
Doc No: 109042327