23 Jul Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application
Abstract
The invention discloses a three-dimensional porous sponge scaffold with a meniscus matrix source and a preparation method and an application. The method comprises the following steps: with meniscus as a raw material, removing cells through mechanical crushing treatment, acetic acid enzyme digestion and a differential centrifugation method to obtain meniscus extracellular matrix slurry; building a three-dimensional porous scaffold through freeze-drying; and carrying out crosslinked reinforcement on the scaffold through ultraviolet and chemical methods. The prepared three-dimensional porous sponge scaffold with the meniscus matrix source has a controllable microstructure, appropriate degradation rate, good biocompatibility and certain biomechanical strength, is beneficial to cell adhesion, uniform distribution of seed cells to enter into the scaffold and proliferation migration growth, is free of immunological rejection when implanted in vivo, can be widely applied to the field of meniscus and cartilage tissue engineering, and has a good clinical application prospect.
Year: 2016
Country: CN
Doc No: 105903079