23 Jul Three-dimensional bracket preparation method for tissue engineering based on supercritical fluid technology
Abstract
The invention discloses a three-dimensional bracket preparation method for tissue engineering based on a supercritical fluid technology. The three-dimensional bracket comprises the following steps: dissolving a polymer to be prepared into a porous bracket into a solvent to prepare a solution or a suspension solution with a certain concentration, wherein the solution or the suspension solution is sufficiently contacted with ScCO2; rapidly dissolving the ScCO2 into the solution or the suspension solution to separate the polymer solution or the suspension solution; separating the solution or the suspension solution into a polymer rich phase and a polymer lean phase which can form a metastable balance; meanwhile, rapidly dissolving the solvent in the solution or the suspension solution into the ScCO2; discontinuously carrying out glass transition and curing on the polymer rich phase to form a porous material; forming a crystal nucleus by the polymer lean phase and enabling the crystal nucleus to grow gradually to fully fill gaps in the porous material; and circularly introducing CO2 to wash and dry the porous material and continuously conveying the solvent into an ScCO2 phase to be brought out of the system, so that the single-component or multi-component or composite-activity porous bracket for the tissue engineering which has no solvent residues is obtained.
Year: 2012
Country: CN
Doc No: 102697579