23 Jul Three-dimensional bone tissue engineering scaffold material with different stiffness and preparation method of material
Abstract
The invention discloses a three-dimensional bone tissue engineering scaffold material with different stiffness and a preparation method of the material. The method comprises the following steps: firstly, mixing collagen with hydroxyapatite according to different ratios, so as to form different concentration gradients of collagen/hydroxyapatite mixed emulsions; immersing the fabricated acellular bone into different concentrations of mixed solutions; reperfusing and lightly vibrating, so as to form an even collagen/hydroxyapatite coating; finally freezing and drying and carrying out crosslinking treatment to obtain the three-dimensional bone tissue engineering scaffold material with different stiffness. Different stiffness of substrates can be formed on the scaffold surface under the condition that the three-dimensional scaffold microstructure is not changed, the scaffold material has good biocompatibility, proliferation and differentiation of different stem cells are facilitated, and the material is low in cost and easy to fabricate.
Year: 2014
Country: CN
Doc No: 103893824