23 Jul Thermosensitive hydrogel based on spider silk protein
Abstract
The invention relates to a thermosensitive hydrogel based on spider silk protein, belonging to the field of biotechnology. The thermosensitive hydrogel is prepared through the steps that nucleotide sequences of c-terminus domain (CTD) and a derived peptide segment thereof of spider silk protein which are artificially designed are connected to pET-19b plasmid vector seamlessly, are expressed by escherichia coli, and then separated and purified, and finally the hydrogel is formed through corresponding temperature control. According to the invention, the hydrogel with dense bore diameter and elasticity modulus being 10<5>Pa is prepared from the CTD and the derived peptide segment thereof of the spider silk protein, and specifically, the hydrogel can be applied to medical field, such as tissue engineering.
Year: 2015
Country: CN
Doc No: 105031723