SYNTHESIS OF NANOTOPOGRAPHIC BIOMIMETIC MEMBRANES FOR TISSUE CULTURE, ENGINEERING AND PROSTHETICS APPLICATIONS

SYNTHESIS OF NANOTOPOGRAPHIC BIOMIMETIC MEMBRANES FOR TISSUE CULTURE, ENGINEERING AND PROSTHETICS APPLICATIONS

Abstract

The present invention provide methods for preparing nanostructured membranes. The methods include: providing a substrate with a charged silanized surface; forming a multilayered membrane containing at least two polyelectrolytes; inducing polyelectrolyte phase separation; crosslinking the multilayered membrane; and covalently linking the multilayered membrane to the silanized surface. Methods for fabricating membrane replicas are also disclosed, as well as devices such as cell- and tissue-culture substrates that contain the membranes and membrane replicas. Resulting materials exhibit topographic features and compliance of the extracellular matrix in vivo.

Year:  2013

Country:  US

Doc No:  2013211542

 

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