23 Jul Sudan grass somatic culture high-frequency plant reproducing technology
Abstract
The present invention relates to a Sudan grass somatic cell culture high-frequency plant regeneration technique, belonging to the field of biological technology, providing practical tissue culture technique for Sudan grass cell engineering and gene engineering. The granular embryonic callus is applicable for developing agrobacterium mediated gene conversion work. The young ear of Sudan grass is explant material, the callus induction culture medium and successive transfer culture medium are basic culture medium + 2.4 ¿C D 3mg/L + KT 0.2 mg/L; and the green seedling differentiation culture medium is basic culture medium + l- BA 2mg/L +NAA 0.01 mg/L. The induction frequency of granular callus is 80%-90%, the callus green seedling differentiation frequency is up to above 60% and the transplanted survival rate of regeneration plant is about 70%-80%.
Year: 2006
Country: CN
Doc No: 1775005