23 Jul Small-molecular compound combination and method for inducing differentiated cells to prepare vascular smooth muscle cells by using small-molecular compound combination
Abstract
The invention discloses a small-molecular compound combination and a method for inducing differentiated cells to prepare vascular smooth muscle cells by using the small-molecular compound combination.The small-molecular compound combination includes a first-stage small-molecular compound and a second-stage small-molecular compound used separately according to time sequence, wherein the first-stage small-molecular compound includes at least one of DNMT inhibitors and lysine deacetylases inhibitors; the second-stage small-molecular compound includes WNT/beta-catenin agonists, cAMP agonists andTG F-beta receptor inhibitors. Through staged induction of the small-molecular compounds, the differentiated cells can be differentiated into the vascular smooth muscle cells, precision quality control can be achieved in each step, and standardization operation and large-scale production are facilitated. The method provided by the invention has wide donor sources, a patient himself can be used asa donor, and the vascular smooth muscle cells needed for basic research, clinical treatment, or tissue engineering production can be obtained in a relatively short period of time.
Year: 2018
Country: CN
Doc No: 108060119