23 Jul Small-molecular compound combination and method for inducing differentiated cells to prepare osteoblasts by using small-molecular compound combination
Abstract
The invention discloses a small-molecular compound combination and a method for inducing differentiated cells to prepare osteoblasts by using the small-molecular compound combination. The small-molecular compound combination includes a first-stage small-molecular compound and a second-stage small-molecular compound used in a staged manner according to time sequence, wherein the first-stage small-molecular compound includes TGF-beta receptor inhibitors, WNT/beta-catenin agonists and cAMP agonists; the second-stage small-molecular compound includes lysine deacetylases inhibitors, TGF-beta receptor inhibitors, PKC inhibitors, WNT/beta-catenin agonists and cAMP agonists. Through phased induction of the small-molecular compounds, the differentiated cells can be differentiated into the osteoblasts, precision quality control can be achieved in each step, and standardization operation and large-scale production are facilitated. The method provided by the invention has wide donor sources, a patient himself can be used as a donor, and the osteoblasts needed for basic research, clinical treatment, or tissue engineering production can be obtained in a relatively short period of time.
Year: 2018
Country: CN
Doc No: 108060121