23 Jul Small-bore artificial blood vessel and preparation method thereof
Abstract
The invention relates to a small-bore artificial blood vessel and a preparation method thereof, and belongs to the technical field of tissue engineering. A vessel wall solution and a solidifying agentsolution are introduced in an artificial vessel preparation device through an extrusion method; a solidified blood vessel is transferred into a cell culture medium, human umbilical vein endothelial cells are introduced into the cell culture medium, and the small-bore artificial blood vessel is obtained through culturing; an artificial blood vessel preparation device is a coaxial sleeve, an outercylinder and an inner cylinder of the coaxial sleeve are of hollow structures,, the inner cylinder is higher than the outer cylinder; the top end of the outer cylinder is in closed connection with theinner cylinder, the top of the inner cylinder is open, the bottoms of the outer cylinder and the inner cylinder are open, and a leading pipe is arranged on the side wall of the outer cylinder; an open container containing a solidifying agent is placed below an artificial blood vessel preparation device body, and the preparation device body is located above the open container; the artificial bloodvessel wall solution is introduced from the leading pipe of the outer cylinder, and the solidifying agent is introduced from the top of the inner cylinder. The prepared artificial blood vessel same as the real blood vessel has certain toughness, strength, micromolecule permeability and high biofidelity.
Year: 2019
Country: CN
Doc No: 109731139