23 Jul Simple and convenient method for inoculating cell for scaffold material of tissue engineering
Abstract
The invention provides a simple, convenient and effective method for realizing three-dimensional uniform inoculation of seed cells on a porous scaffold material of biological engineering. The method is characterized by comprising the following steps: using a clinically conventional sterilized syringe as an inoculating tool; putting a required biological scaffold material into the syringe from the back part thereof; sucking cell suspension, required for inoculating, with a corresponding number of cells; preserving a certain amount of air in the syringe; sealing the opening of the syringe; withdrawing the syringe; and enabling the cell suspension to permeate into air gaps of a porous material by using a formed negative pressure so as to realize the initial-stage uniform distribution and inoculation of the seed cells in the scaffold material. Compared with the method for realizing the initial-stage three-dimensional uniform inoculation of the scaffold material in the conventional tissue engineering practice, the method of the invention has the advantages of simplicity, convenience, easy implementation, and capability of achieving the effect of initial-stage three-dimensional uniform inoculation similar to special equipment without purchasing a special instrument with a large amount of funds. The inoculated cells are not damaged because the acting time of the negative pressure is short and the pressure is low.
Year: 2010
Country: CN
Doc No: 101735984