23 Jul Sericin/nano-hydroxyapatite tissue engineering bone graft as well as preparation method and application thereof
Abstract
The invention belongs to the technical field of tissue engineering, and discloses a construction (preparation) method of a sericin/nano-hydroxyapatite tissue engineering bone graft containing bone morphogenetic protein-2 and an application thereof. The graft comprises growth factors, a scaffold material and optional seed cells, wherein the seed cells are adhered to the scaffold material, a cell carrier compound with a space structure and biological activity is formed, the scaffold material is a composite scaffold material composed of sericin (SS) and nano-hydroxyapatite (nHAP), the sericin wraps a growth factor containing human recombinant bone morphogenetic protein-2, and bone marrow mesenchymal stem cells are adhered to the composite scaffold material. The pore diameter, degradation speed and biomechanical strength of the scaffold can be adjusted according to the ratio of SS to nHAP. The constructed bionic artificial tissue engineering bone can be used for repairing bone grafts withlarge-section bone defects, and animal experiments prove that the bionic artificial tissue engineering bone can well repair the large-section bone defects.
Year: 2020
Country: CN
Doc No: 110665055