23 Jul Separation culture technology for animal muscle satellite cells
Abstract
The invention belongs to the technical field of cytobiology and tissue engineering interdisciplines and discloses a separation culture technology for animal muscle satellite cells. After rat 1% pentobarbital sodium intraperitoneal anesthesia (40 mg/kg) succeeds, a fore limb triceps is taken out under the sterile condition, immediate flushing is conducted three times with D-Hank’s liquid, and separation culture is conducted with an enzyme digestion method. The separation culture technology has the advantages of achieving double-enzyme digestion, step-by-step centrifugation, multi-mesh filtration, double-face adherence and the like, and is easy and convenient to operate and high in culture purity.
Year: 2016
Country: CN
Doc No: 105505861