23 Jul Separation culture method for cartilage stem cells
Abstract
The invention provides a separation culture method for cartilage stem cells. In the method, the cartilage stem cells are screened from cartilage tissue cells; fibroblast-like cells maintained under the culturing condition of a specific culture medium have the capabilities of differentiating bones, cartilages and fat; and compared with adult cells, the cartilage stem cells have good amplification capabilities, and can pass on for over 15 generations without changing the original characteristics after being obtained from a primary generation. In the method, the cartilage stem cells are obtained by screening and purifying under a specific culturing condition, so that the content of the cartilage stem cells is over 95 percent. A technology disclosed by the invention contributes to harvesting cartilage tissue specific stem cells, and can be applied to bone and cartilage tissue engineering.
Year: 2012
Country: CN
Doc No: 102399745