23 Jul Separating culture and use for fatty mesenchymal dry cell
Abstract
The invention is about the separating culture method of the stem cell between the lipid and the applying of it. First to take the human fat tissue, next to digest by the collagen enzyme, then to through the gradient centrifugation and the filting, last to amplifying culture in vitro culture which includes the DMEM/F12, EFG and the FCS. The stem cell has the CD11a-, the CD31-, the CD34-, the CD45-, and the HLA-DR-, the phenotype of the CD44+ or the FIK-1+. The invention also opened the new use of the stem cell; it provided the new choice for the tissue engineering, the seed cell resource and the stem cell group.
Year: 2006
Country: CN
Doc No: 1778905