23 Jul Scaffold material for in vitro osteogenesis induced differentiation of mesenchymal stem cells, and application of scaffold material
Abstract
The invention discloses a scaffold material for in vitro osteogenesis induced differentiation of mesenchymal stem cells, and application of the scaffold material. The scaffold material is characterized in that a porous silk protein scaffold is taken as a main body, the scaffold is enveloped with organic silicon by means of biological silicification, and the porous silk protein scaffold enveloped with the organic silicon is taken as a culture carrier for the in vitro osteogenesis induced differentiation of human marrow mesenchymal stem cells; the human marrow mesenchymal stem cells are taken as seed cells and are inoculated onto the porous silk protein scaffold enveloped with the organic silicon according to certain cell density, and the porous silk protein scaffold enveloped with the organic silicon is placed in an osteogenesis induced differentiation micro-environment for culturing. A three-dimensional osteogenesis induced differentiation system established by using the scaffold material not only can be used for directionally inducing the human marrow mesenchymal stem cells to differentiate into osteoblasts in bone tissue engineering so as to enable the osteoblasts with stable traits to be more efficiently obtained, but also can provide important theoretical guidance for the research of the bone tissue engineering so as to provide new ideas for the clinical treatment of osteoporosis, bone injury and other orthopedic diseases.
Year: 2017
Country: CN
Doc No: 107446885