23 Jul ROTATIONAL DUAL CHAMBER BIOREACTOR: METHODS AND USES THEREOF
Abstract
A rotational dual chamber bioreactor for cell culture in bi- and multilayered scaffolds, in the context of tissue engineering (TE) and regenerative medicine strategies is disclosed which comprises at least two dual culture chambers, a multiposition magnetic stirrer plate, and at least two flow pumps which can be coupled to a monitor device to register biochemical parameters such as pH, pO2, glucose and urea sensors and physical parameters like pressure. The dual culture chambers have a central barrier with a hole to insert the bilayer scaffold, such chamber is used to culture cells with different conditions in each compartment of the chamber, avoiding medium exchange. Each chamber can rotate 180° (horizontal), being the rotations per minute controlled independently. The rotation is promoted by magnetic stirrers, having magnets performing the attraction between the bottom part of the dual chamber and the stirring position of the plate. The multiposition stirrer plate is adapted dimensionally to the requirements of the culture plate dimensions used, and also allows 180° vertical rotation. Each dual chamber has detachable caps for the top and the bottom chambers. The top cap has the possibly to compress the scaffold and thus providing a compressive stimulus with torsion simultaneously. This compression is applied by attaching a top plate adapted to fit the top caps of each dual chamber.
Year: 2016
Country: US
Doc No: 2016024453