ROTATIONAL DUAL CHAMBER BIOREACTOR: METHODS AND USES THEREOF

23 Jul ROTATIONAL DUAL CHAMBER BIOREACTOR: METHODS AND USES THEREOF

Abstract

Rotational dual chamber bioreactor for cell culture in bi- and multilayered scaffolds for tissue engineering and regenerative medicine comprising at least two dual culture chambers, a multiposition magnetic stirrer plate, and at least two flow pumps which can be coupled to a monitor device to register biochemical parameters using pH, p0 2, glucose and urea sensors and physical parameters like pressure. The dual culture chambers have a central barrier with a hole to insert the bilayer scaffold. This kind of chamber is used to culture cells with different conditions in each compartment of the chamber, avoiding medium exchange. Each chamber can rotate 180° horizontally or vertically, being the rotations per minute controlled independently. Each dual chamber has detachable caps for the top and the bottom chambers. The top cap can compress the scaffold providing a compressive stimulus with torsion simultaneously.

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