23 Jul Rooting culture method for pear test-tube plantlets and culture medium
Abstract
The invention provides a rooting culture method for pear test-tube plantlets and a culture medium, and belongs to the technical field of plant cell engineering. The rooting culture method comprises the following steps: carrying out multiplication culture, strong seedling culture, induced rooting culture and large-amount rooting culture in sequence based on pear test-tube plantlets; the induced rooting culture step comprises the following sub-steps: sequentially inoculating tissue culture seedlings obtained after the strong seedling culture into a rooting culture medium A and a rooting culture medium B to be cultured to obtain rooting seedlings; and the large-amount rooting culture step comprises the following sub-steps: inoculating the rooting seedlings into a rooting culture medium C to be cultured, wherein the rooting culture medium A is a culture medium obtained by adding IAA, BA and NAA into a 1/4QL culture medium, the rooting culture medium B is a 1/4QL culture medium and the rooting culture medium C is a liquid culture medium obtained by adding IBA into a 1/3MS culture medium. The invention further provides the culture medium used in the method. By using the rooting culture method provided by the invention, the rooted test-tube plantlets with the relatively high multiplication rate, rooting rate, average rooting quantity and the relatively long average root length can be obtained.
Year: 2014
Country: CN
Doc No: 104186321