23 Jul Renaturation method of genetic engineering tissue-type plasminogen activator recombinant isomer (nt-PA) inclusion body
Posted at 13:32h
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Abstract
The invention provides a novel process method for realizing industrialization of correct folding renaturation of an inclusion body formed by an escherichia coli expressed tissue-type plasminogen activator recombinant isomer (nt-PA). According to the method, through developed unique denaturation and renaturation systems, a large number of mismatched disulfide bonds in the nt-PA inclusion body are reopened and correct disulfide bonds are formed, so that the renaturation rate is increased, the yield of the nt-PA is ultimately increased, the cost is reduced, and the novel process method can be applied to industrial production.
Year: 2019
Country: CN
Doc No: 110387365