Recombined anti-human TNF alpha gene engineering anti-bogy and its preparing method

Recombined anti-human TNF alpha gene engineering anti-bogy and its preparing method

Abstract

Of the anti-human TNF alpha engineering antibody, the gene sequence has a record number of AF252545 and AF 262753 in Genebank. The preparation process includes the main steps of: reverse transcription polymerase chain reaction to obtain the whole sequence and partial sequence of required anti-human TNF alpha gene engineering antibody; gene engineering measure to obtain the recombined expressed product of anti-human TNF alpha gene engineering antibody; and purification of the product to obtain recombined anti-human TNF alpha gene engineering antibody with high activity. The antibody of presentinvention has the functions of neutralizing TNF alpha toxicity and protecting tissue.

Year:  2001

Country:  CN

Doc No:  1280190

 

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