23 Jul Recombinant thymosin protein PaTHY1 of Periplaneta americana and expression method thereof
Abstract
The invention discloses a recombinant thymosin protein PaTHY1 of Periplaneta americana and an expression method thereof, belonging to the field of biotechnology. The expression method comprises the following steps: (1) extracting RNAs from the tissue of Periplaneta americana and carrying out inverse transcription on the RNAs to obtain cDNAs; (2) synthesizing primers; (3) with the cDNAs as a template, carrying out PCR to obtain a coding gene fragment of the thymosin protein of Periplaneta americana; (4) cloning the coding gene fragment into a prokaryotic expression vector for transformation ofhost bacterium, carrying out culturing on an obtained recombinant engineering bacterium and inducing the recombinant engineering bacterium to express the target protein; and (5) purifying the target protein by using affinity chromatography so as to obtain the recombinant thymosin protein PaTHY1 of Periplaneta americana. The recombinant protein is obtained through genetic engineering for the firsttime, overcomes the problems of low content and difficult purification of thymosin in complex chemical components of Periplaneta americana, and lays a foundation for research on the pharmacodynamic functions of thymosin from Periplaneta americana.
Year: 2018
Country: CN
Doc No: 107759684