23 Jul Recombinant lentivirus for carrying out targeted induction on osteogenic differentiated cell apoptosis as well as preparation method and application thereof
Abstract
The invention discloses recombinant lentivirus for carrying out targeted induction on the osteogenic differentiated cell apoptosis as well as a preparation method and application thereof. A recombinant lentivirus genome contains an Apoptin gene expression box which contains an Osterix gene promoter, an Apoptin gene and a terminator, and the Apoptin gene expression is regulated and controlled by the Osterix gene promoter; the recombinant lentivirus takes a pan-osteogenic cell peculiar expression gene Osterix promoter as a promoting element, expresses Apoptin for inducing the osteogenic differentiated cell apoptosis in osteogenic differentiated cells, does not play a role on chondrogenic differentiated cells and normal cartilage cells, can be prepared into medicines for inducing the osteogenic differentiated cell apoptosis, carries out the treatment on residual osteogenic differentiated cells in seed cells after the osteogenic induced differentiation treatment and ensures a single chondrogenic differentiation path of the seed cells, thereby providing a favorable seed cell for the establishment of tissue engineering cartilage; and the preparation method of the recombinant lentivirus is simple, convenient, rapid and low in cost.
Year: 2010
Country: CN
Doc No: 101787359