23 Jul Recombinant homo sapiens kallikrein 1 as well as coding agene and preparation method thereof
Abstract
The invention provides recombinant homo sapiens kallikrein 1 as well as a coding gene thereof and an expressing, purifying and inclusion body refolding method thereof, belonging to the field of the bio-genetic engineering. The KLK1 has therapeutical effect on various diseases such as cardiovascular and cerebrovascular diseases and diabetic complication; however, KLK1 extracted from an animal tissue or human fluid is lower in yield and possibly involves a safety problem. According to the invention, an escherichia coli expression system is adopted for carrying out heterologous expression on a codon-optimized recombinant homo sapiens kallikrein 1 gene. Besides, in order to solve a problem that the homo sapiens kallikrein 1 in a prokaryotic expression system mostly expresses in the form of inclusion body, the invention further provides a method of refolding and purifying the recombinant homo sapiens kallikrein 1 inclusion body, wherein the homo sapiens kallikrein 1 has purify of over 95% by being further purified, and has higher activity.
Year: 2014
Country: CN
Doc No: 103710367