23 Jul Recombinant expression vector expressing human pancreatic tissue kallikrein gene and prepn of human pancreatic tissue kallikrein
Abstract
The present invention relates to secretion or non-secretion recombinant vector carrying mature protein gene coding human pancreatic kallikrein and its transformed host cell. The present invnetion also relates to the gene engineering technology method of preparing recombinant human pancreatic kallikrein. The expression vector of the present invention can express effectively protein product with pancreatic kallikrein activity and has fused His affinity sequence and the expression product containing the affinity sequence. During separation and purification, His affinity resin may be used in chromatographic affinity purification. The present invention lays the foundation for developing humanized tissue kallikrein gene engineering product and medicine.
Year: 2002
Country: CN
Doc No: 1384199