23 Jul Recombinant adeno-associated virus increasing targeted transduction efficiency of adeno-associated virus, and application of recombinant adeno-associated virus
Abstract
The invention discloses recombinant adeno-associated virus increasing targeted transduction efficiency of adeno-associated virus, and application of the recombinant adeno-associated virus. The recombinant adeno-associated virus contains modified VP2 protein of the adeno-associated virus and primary VP1 protein, primary VP2 protein and primary VP3 protein of the adeno-associated virus, wherein the modified VP2 protein of the adeno-associated virus is formed in the way that a section of FLAG tag sequence is inserted into N terminus of the VP2 capsid protein. The invention further discloses a construction method of the recombinant adeno-associated virus and the application of the recombinant adeno-associated virus in gene therapy and in preparation of tissue engineering scaffold materials. The recombinant adeno-associated virus disclosed by the invention contains FLAG epitope, thus being capable of effectively infecting target cells and enhancing transduction efficiency. Compared with the conventional virus vector, the recombinant adeno-associated virus has higher transduction efficiency and better targeting performance, thus being applicable to multiple aspects of the gene therapy, construction of virus delivery systems, the preparation of the tissue engineering scaffold materials, and the like.
Year: 2012
Country: CN
Doc No: 102618507