23 Jul Rat pancreas islet epithelioid stem cell system
Abstract
The invention relates to a rat pancreas islet epithelioid stem cell system and belongs to the field of animal cell (tissue) engineering. The rat pancreas islet epithelioid stem cell is from a normal adult rat pancreas islet tissue, cultured in vitro, and can grow in a clonal manner; moreover, the cell has a plurality of differentiation potentials, specifically cell adherent culture is polygonal and epithelioid, cell nucleus is oval, and nucleolus is a single nucleolus or amphinucleolus; in cell clonal growth, cells grow slowly on the first day after being inoculated, enter a logarithm growth period on the second day to the fourth day and enter a platform period on the fifth day to the sixth day, and multiplication capacity is reduced on the seventh day; the cells are normal diploid cells with a chromosome number 2n equal to 42; an octamer transcription factor 4, a paired-gene cassette 4, paired-gene cassette 6, a neurogenic factor 3 and nidogen are expressed on protein level. The rat pancreas islet epithelioid stem cell system has the plurality of differentiation potentials, is directionally induced in vitro to differentiate and form nerve cells, fat cells and osteoblast, and is free of oncogenicity.
Year: 2014
Country: CN
Doc No: 103881961