23 Jul Pyrus stem tip tissue culture rapid propagation method
Abstract
The invention belongs to the technical field of plant cell engineering, and discloses a pyrus stem tip tissue culture rapid propagation method. The method adopts 1/2MS as a basic culture medium, an explant is sterilized, and a regenerated plant is acquired through bud induction, multiplication culture and subculture of cluster buds and rooting culture. The method is simple to operate, convenient for drawing materials and low in cost, can acquire a great amount of clone propagated seedlings with consistent hereditary character within a short time, can effectively solve the technical problem of rapid propagation of the root of the pyrus, sets a foundation for the genetic transformation of the pyrus and has great practical value.
Year: 2012
Country: CN
Doc No: 102648698