23 Jul Putuo hornbeam gemma culture medium and tissue culture rapid propagation method
Abstract
The invention relates to a culture medium for carpinus putoensis gemmas which is prepared by a 1/3MS salt, an MT vitamin, an IBA (indolebutyric acid) which is 0mg/l to 60mg/l, an inose which is 50mg/l to 150mf/l, a gelrite which occupies 0.2 percent to 0.4 percent of the total weight of the culture medium and a cane sugar which occupies 1.5 percent to 4.5 percent of the total weight of the culture medium. The culture medium can culture abacterial test-tube gemmas effectively and induce rooting, thereby rapidly culturing and reproducing a mass of regrowth. The process for tissue-culturing and rapidly reproducing carpunus putoensis by using the tissue culture medium comprises the following four steps: the first one is the collection and disinfection of explants, the second one is the culture of abacterial gemmas, the third one is the root induction with binary form and the last one is the domestication of transplants. The invention firstly takes the gemmas as explants to tissue-culture and rapidly reproduce carpinus putoensis saplings, which overcomes the physical disabilities of the tree like bad germination, short life of pollen, low sprout rate and insufficient effective pollination period and provides biological engineering technical measures for the reproduction and preservation of the tree, thereby saving the carpinus putoensis species resource which is almost extinct.
Year: 2009
Country: CN
Doc No: 101402936