23 Jul Purifying and concentrating method for composite bioactive factors and concentrated solution
Abstract
The embodiment of the invention provides a purifying and concentrating method for composite bioactive factors and a concentrated solution. A tissue engineering skin condition culture solution containing the composite bioactive factors is provided and subjected to decontamination treatment to remove substances with the molecular weight larger than 30 kD in the tissue engineering skin condition culture solution, and thus a middle solution containing the composite bioactive factors is obtained; then the middle solution is concentrated through a tangential flow ultrafiltration and concentration assembly to intercept substances with the molecular weight larger than 3 kD in the middle solution, and the concentrated solution containing the composite bioactive factors is obtained. The process is simple, implementation is convenient, various bioactive factors can be purified and concentrated at the same time, and the purifying and concentrating efficiency for the various composite bioactive factors is improved; the obtained composite bioactive factors are high in purity, and the number of varieties of the included composite bioactive factors is large.
Year: 2016
Country: CN
Doc No: 105457102