23 Jul Promoters of pig adipose tissue specific expression gene plin and use thereof
Abstract
The invention belongs to the technical field of animal gene engineering and relates to separation and identification and functional verification of different-length promoter regions of a pig adipose tissue specific expression gene plin. According to the invention, 6 upstream different-length promoters of the pig adipose tissue specific expression gene plin in a pig genome are cloned; nucleotide sequences of the promoters are shown in the formulas of SEQ ID NO: 2 to SEQ ID NO: 7; and a detection result shows that the promoter fragment from -130bp to -910bp and the promoter fragment from -435bp to -896bp have independent promoter activity. The invention also discloses a preparation method of the 6 different-deletion promoter fragments and a corresponding recombinant expression vector, and a use of a dual-luciferase activity detection system in promoter activity analysis.
Year: 2013
Country: CN
Doc No: 103421780