23 Jul Promoter and expression mode identification of rice endosperm specific expression gene
Abstract
The invention belongs to the field of plant genetic engineering. Six upstream promoters (EnP3, EnP3-859, EnP3-678, EnP3-471, EnP3-292 and EnP3-110) with different lengths of the same endosperm specific expression gene (access number: GI 3141524) are cloned from a rice genome; and the nucleotide sequences of the promoters are nucleotide sequence SEQ ID No.1, nucleotide sequence SEQ ID No.2, nucleotide sequence SEQ ID No.3, nucleotide sequence SEQ ID No.4, nucleotide sequence SEQ ID No.5 and nucleotide sequence SEQ ID No.6. Five promoters (EnP3, EnP3-859, EnP3-678, EnP3-471 and EnP3-292) are expressed only in endosperms of transgenic plants, and the expression of the five promoters reduces as the endosperms grow; and the expression of the five promoters can be detected in none of leaf blades, leaf sheaths, stalks, roots, flowers, glumes and embryos. The expression of the promoter EnP3-110 can detected in green tissues such as the leaf blades, leaf sheaths, stalks, and glumes, but not in the roots, flowers and seeds (including embryos and endosperms), so the promoter EnP3-110 is a short green tissue specific expression promoter. The invention also discloses a method for preparing the six promoters and the corresponding expression vectors thereof and the use of the six promoters through the transfer of the six promoters into rice by using transgenic method of agrobacterium rhizogenes mediation.
Year: 2010
Country: CN
Doc No: 101831429