23 Jul Producing method of recombination human fibroblast growth factor (FGF)-18
Abstract
The invention discloses a method for expressing and producing an rhFGF-18. The full-length nucleotide sequence of the FGF-18 is optimally designed and synthesized through bioinformatics means, pET serial carriers and escherichia coli host cells are screened out, engineering bacterial strains with soluble expression and stable inheritance are obtained, a high-density fermentation method, a two-step column chromatography purification method and a corresponding activity detection method are established, and the large-scale production of the rhFGF-18 becomes possible. On this basis, the application of the rhFGF-18 to the fields of skin burn and dermal chronic ulcer treatment, mucosal injury tissue repair, biological beauty, and androgenetic alopecia, alopecia seborrhoeica, alopecia areata and after-chemoradiotherapy alopecia prosthodontic treatment is further discussed.
Year: 2015
Country: CN
Doc No: 105176908