23 Jul Process for fabricating engineering esophagus imitating biochemistry tissue
Abstract
The invention relates to a preparation technique of a biochemically emulated tissue engineering gullet, which is a bio-active molecule adopts electrical weaving method to weave biodegradalbe polyester polymer for a second time, thus obtaining basement membranes of epithelium similar with gullet and an asymmetric porous nano fiber structure arranged in sequence and similar with muscular layer structure, and the fiber is chemically modified to ingraft the surface of the biochemically emulated tissue engineering gullet and facilitate the growth of cells. The porous nano fiber similar with the basement membranes can support the growth of gullet epithelium, while the oriented fiber arranged in sequence is helpful for inducing the oriented growth of gullet smooth muscle cells, and the porous nano fiber can is cultured for 3 to 4 weeks in DMEM wholly culture medium, thus preparing a biochemically emulated tissue engineering gullet with both epithelium and muscular layer. The gullet of the invention completely simulates the normal gullet tissue of human body in microstructure causing the man made gullet constructed in vitro to be much closer to human gullet in structure and functions, and the ingrafted bio-active molecules are beneficial for retaining the original form and bio-functions of the body cells for the cells ingrafted to the bio-active molecules, and can be used for the restoration and construction of diseased gullet.
Year: 2008
Country: CN
Doc No: 101204592