23 Jul Preparation technology of biphasic bone and cartilage tissue engineering scaffold material
Abstract
The invention discloses a preparation technology of biphasic bone and cartilage tissue engineering scaffold material. Firstly chitosan and collagen powder are added into acetate solution and laid in a refrigerator to be preserved; then PLA solution is prepared, and bone dust is put into the PLA solution and is uniformly mixed and stirred; then prepared PLA/bone dust solution is injected into a die to be prefrozen; then collagen/chitosan solution is added into the prefrozen die to be prefrozen, and a scaffold sample is obtained after freeze drying; and finally the bone and cartilage biphasic scaffold conducted to freeze drying is taken out from the die. The preparation technology ensures that a bone layer scaffold has the hardness and the strength approximate to that of natural bone tissue, is not only beneficial to the repair of cartilage tissue, but also convenient for clinically operating and fixing the scaffold; and meanwhile, a cartilage layer scaffold is provided with a multilayer structure containing perpendicular pore canals, is convenient for guiding cells on the scaffold to grow at a state close to that of a natural cartilage laminated structure, and improves the injury repair effect of cartilage.
Year: 2013
Country: CN
Doc No: 102872480