23 Jul Preparation of secoiridoid glycoside complex and application of secoiridoid glycoside complex in improving viability of bone-marrow mesenchymal stem cells
Abstract
The invention belongs to the field of biomedicine and provides preparation of a secoiridoid glycoside complex and application of the secoiridoid glycoside complex in improving the viability of bone-marrow mesenchymal stem cells in order to solve the problems about changes in the cellular morphology, metabolism, proliferation and differentiation potential of bone-marrow mesenchymal stem cells (BMSCs) cultured in vitro, improve the proliferation of the BMSCs cultured in vitro and keep the BMSCs good in differentiation potential. The secoiridoid glycoside complex is prepared by mixing oleuropein, rehmaionoside A and gentiopicroside with a conventional in-vitro cell culture medium in a certain proportion. By means of the secoiridoid glycoside complex, the proliferation capacity of the BMSCs can be improved, short-term proliferation is promoted, so that a lot of cells are obtained to be used for transplantation for treatment, and moreover, the survival rate of the transplanted cells in vivo can be improved. When the complex acts on the BMSCs for a long time, the oxidative-damage-resistant ability of the cells can be improved, and the BMSC differentiation tending to neurons is promoted without changing the biological characteristics of the BMSCs. A method is provided for the wide application of the BMSCs in research of medical tissue engineering.
Year: 2017
Country: CN
Doc No: 107090430